Lect1.2 Big Data Challenge
Sequencing in 2001 :
Genome Center for Sequencing in 2001:
| Aspect | Production | Sequencing |
|---|---|---|
| Location | ||
| - Room of Equipment | Yes | |
| - Sample Preparations | Yes | |
| Number of People | 35 | 10 |
| Duration (Weeks) | 3-4 | |
| Sequencing Details | ||
| - Capillary Sequences | 74x | |
| - Runs per Day | 15-40 | |
| - Mb per Instrument per Day | 1-2 | |
| - Total Capacity per Day (Mb) | 120 |
Sequencing in 2007
| Aspect | Production | Sequencing |
|---|---|---|
| Equipment | 1x cluster station | 1x Genome Analyzer |
| Number of People | 1 | Same Person |
| Duration (Days) | 1 | 3-5 days per run |
| Data Output per Day (Gb) | Not applicable | 0.5Gb per instrument |
Few Machines for this:
- ISeq 100
- MiniSeq
- MiSeq SEries
- NextSeq 550 Series
- NextSeq 2000
- NovaSeq 6000
Initialy human Genome cost 30 billions over 30 years now it can cost couple of thousands, in 2001 it would cost 100 million.
Every time you sequence Genome it's terabytes and terabytes of data, there are algo which are need to process quicly if take raw data it will take a lot of sotage space. Algorith for mutation 99 percent of our geno is repetative so there is no need to save the smilira seq (opens in a new tab)
Example of Angelina Jolie's preventative Double Mastectomy inherated risk of developing cancer. This can also helps to check how much of the asian affrican boold you have which can heritate certain diesase.